What are Cre-driver mice?
Cre-driver lines: A special type of transgenic mouse As different neurons have different cellular machinery to activate and express certain genes, Cre-recombinase was targeted to specific neuronal (or other brain cell types) with this in mind.
What is LysM Cre mice?
2.1. LysM-Cre (MGI: 1934631) Lysozymes are antimicrobial enzymes widely produced by the innate immune cells in eukaryota. As a type of glycoside hydrolase, lysozymes function to cleave the peptidoglycan of bacterial cell wall as their antimicrobial mechanism (9).
Where is Cre in a neuron?
Cre is expressed in layer 6 corticothalamic neurons in an ntsr1 BAC-Cre line (B, Ntsr1). Clearly labeled are the projection axons of these neurons, which terminate in the dorsal thalamic nuclei.
What is Cre expression?
Regulated Cre expression – placing Cre downstream of promoters that are active only in certain cell or tissue types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in specified cells or at specified times.
How do Cre lines work?
Cre recombinase proteins bind to the first and last 13 bp regions of a lox site forming a dimer. This dimer then binds to a dimer on another lox site to form a tetramer. The double stranded DNA is cut at both loxP sites by the Cre protein. The strands are then rejoined with DNA ligase in a quick and efficient process.
What are driver lines?
These Cre “driver” lines can be used to generate conditional mutations that are activated in distinct cell types, tissues or time points, or inducible mutations that are activated through administration of a drug.
How do you get Cre?
CRE are usually spread person to person through contact with infected or colonized people, particularly contact with wounds or stool (poop). This contact can occur via the hands of healthcare workers, or through medical equipment and devices that have not been correctly cleaned.
What is ROSA26 locus?
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. It was first isolated in 1991 in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus. The human homolog of the ROSA26 locus has been identified.
Is Cre a promoter?
First, Cre-driver strain is generated in which Cre recombinase is expressed by a promoter that specifically targets the cell or tissue of interest. Second, loxP flanked (floxed) DNA containing mouse strain is needed to be generated.
What is crecre driver mice?
Cre Driver Mice Targeting Macrophages The Cre/loxP system is a widely applied technology for site-specific genetic manipulation in mice. This system allows for deletion of the genes of interest in specific cells, tissues, and whole organism to generate a diversity of conditional knockout mouse strains.
How to generate spatiotemporally controlled mutant mice using Cre-loxP?
To generate the spatiotemporally controlled mutant mice, two elements are needed in the Cre-loxPsystem [10,11,12,13]. First, Cre-driver strain is generated in which Cre recombinase is expressed by a promoter that specifically targets the cell or tissue of interest. Second, loxPflanked (floxed) DNA containing mouse strain is needed to be generated.
What is the Cre/loxP system?
The Cre/loxP system is a widely applied technology for site-specific genetic manipulation in mice. This system allows for deletion of the genes of interest in specific cells, tissues, and whole organism to generate a diversity of conditional knockout mouse strains. Additionally, the Cre/loxP system …
How to generate conditional knockout mice from loxpflanked (floxed) mice?
Second, loxPflanked (floxed) DNA containing mouse strain is needed to be generated. Conditional knockout mice are then generated by breeding the Cre-driver strain with a floxed mouse strain (Figure 1B).