How do you make a glucose oxidase solution?
Substrate Solution: weigh 10 mg of 4-aminoantipyrine and 340 mg of phenol into a 100 ml volumetric flask. Add 10 ml of deionized water to dissolve the reagents. Then add 75 ml of glucose/benzoic acid solution (Reagent B) and dilute to 100 ml with deionized water. Store at +4°C.
What is the oxidation reaction of glucose that happens in the testing process?
Oxidation of Glucose: The enzyme glucose oxidase catalyzes the oxidation of beta-D-glucose to D-gluconic acid. The alpha-D-glucose is rapidly converted to the beta form so that all of the glucose is measured at one time.
What are the products produced by the action of glucose oxidase?
Glucose Oxidase EC 1.1. Glucose oxidase catalyzes the oxidation of b-d-glucose to form d-glucono-1,5-lactone and hydrogen peroxide. Glucose oxidase has a gluten-strengthening effect by inducing the formation of protein–protein bonds that strengthen the protein network and thereby strengthen and stabilize the dough.
Is glucose oxidase used in Elisa?
A glucose oxidase based colorimetric ELISA was developed for naked-eye detection of AFB1. The BCP color change was regulated by glucose oxidase oxidization of glucose into gluconic acid. The cutoff limit of colorimetric ELISA was 100 pg/mL.
What is glucose oxidase reagent?
Glucose oxidase/peroxidase reagent along with O-Dianisidine was used for the determination of blood and urinary glucose, especially the presence of fructose for both research and clinical purpose. It may be used for determining glucose in plasma or serum.
What two things are produced from glucose?
When oxidized in the body in the process called metabolism, glucose produces carbon dioxide, water, and some nitrogen compounds and in the process provides energy which can be used by the cells.
What is responsible for the production of glucose?
The liver both stores and produces sugar… The liver acts as the body’s glucose (or fuel) reservoir, and helps to keep your circulating blood sugar levels and other body fuels steady and constant. The liver both stores and manufactures glucose depending upon the body’s need.
Is glucose oxidase specific for glucose?
Glucose oxidase is very specific for glucose in the initial oxidation reaction, but can use many different compounds as the final electron acceptor, not just oxygen.
Why is the oxidase test used?
The oxidase test is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain. (note: All bacteria that are oxidase positive are aerobic, and can use oxygen as a terminal electron acceptor in respiration.
How does glucose react with glucose oxidase?
Glucose oxidase catalyzes a reaction in which glucose sugar is broken down into hydrogen peroxide and gluconolactone. This reaction simultaneously consumes the glucose and exhibits significant activity against harmful organisms due to the production of hydrogen peroxide.
Does E. coli test positive for oxidase?
The test result of the oxidase test for E. coli was negative and matched the expected result (Holt 179). The bacteria of the Genus Escherichia are facultative anaerobes. They can use aerobic respiration or fermentation metabolic pathways. The test result of the oxidase test for P. aeruginosa was not as expected.
Is ecoli oxidase positive?
Escherichia coli (commonly abbreviated E. coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans.
Does Staphylococcus aureus ferment glucose?
The ability of Staphylococcus aureus to ferment sugar as main food sources is important. Most isolates of Staphylococcus aureus have the ability to produce acid from various sugar types, such as glucose, mannitol, mannose , terhalose, maltose and sucrose.