How does the Alamar blue assay measure cell viability?
alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent.
How do you make Alamar blue solution?
- Dissolve alamar blue in PBS (pH 7.4) to 0.15 mg/ml.
- Filter-sterilize the resazurin solution through a 0.2 μm filter into a sterile, light protected container.
- Store the resazurin solution protected from light at 4°C for frequent use or at -20°C for long term storage.
What is the Alamar blue assay?
alamarBlue is a cell proliferation assay that provides a rapid, sensitive, and economical way to quantitatively measure cell proliferation and cytotoxicity in various human and animal cell lines. It can also be used to study mycobacteria, bacteria, and fungi.
What is PrestoBlue?
PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. This color change can be detected using fluorescence or absorbance measurements.
What is Alamar?
Alamar Blue Cell Viability Assay (6 Citations) AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent.
Is Alamar blue light sensitive?
alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature.
What is the principle of MTT assay?
The MTT assay is a colorimetric assay for measuring cell metabolic activity. It is based on the ability of nicotinamide adenine dinucleotide phosphate (NADPH)-dependent cellular oxidoreductase enzymes to reduce the tetrazolium dye MTT to its insoluble formazan, which has a purple color (Fig. 10.1).
What is an LDH assay?
The LDH assay, also known as LDH release assay, is a cell death / cytotoxicity assay used to assess the level of plasma membrane damage in a cell population.
What is resazurin assay?
The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity. It is a fast, simple, accurate and homogeneous / no-wash high throughput assay that can be used to monitor cells for up to 24-48 hours.
How does resazurin reduction assay work?
The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.
What is toxic to cells?
Cytotoxicity is the quality of being toxic to cells. Examples of toxic agents are an immune cell or some types of venom, e.g. from the puff adder (Bitis arietans) or brown recluse spider (Loxosceles reclusa).
What is cell viability assay?
Cell viability is a measure of the proportion of live, healthy cells within a population. Cell viability assays are used to determine the overall health of cells, optimize culture or experimental conditions, and to measure cell survival following treatment with compounds, such as during a drug screen.
What is the alamarblue HS cell viability reagent?
The alamarBlue HS Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi. Measuring changes in cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs.
What is the cost of alamarblue?
Invitrogen™ alamarBlue™ HS Cell Viability Reagent alamarBlue™ HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability $253.00 – $516.00
What is the cell viability reagent?
alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability.
Is the alamarblue assay quantitative or qualitative?
alamarBlue is Quantitative. The alamarBlue assay is quantitative with respect to time and dose (Figure 1) as seen by the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator.