What is a good Caco-2 permeability?

What is a good Caco-2 permeability?

Most drugs are not especially permeable through Caco-2 cells, with the median Papp value being some 16 ⋅ 10−6 cm s−1. This value is considerably lower than those (1,310 and 230 ⋅ 10−6 cm s−1) recently used in some simulations that purported to show that Papp values were too great to be transporter-mediated only.

What is a Caco-2 assay?

Caco-2 permeability assay to investigate intestinal permeability. Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs.

Where are Caco-2 cells from?

colon carcinoma
The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine.

What is the name of Caco-2?

Calcium–cobalt
Calcium–cobalt (1/2)

What is Papp permeability?

Apparent permeability (Papp) based on the flux of a molecule across a barrier of known molecular weight is a measure of the integrity of the in vitro BBB model.

What are the advantages of using a Pampa assay over a caco2 assay?

It is high throughput, low cost and is amenable to automation. This method has been shown useful in assessing transmembrane, non-energy dependent, diffusion of drugs such that reasonable predictability with in vivo (passive) absorption is possible.

What do Caco-2 cells do?

Caco-2 cells express tight junctions, microvilli, and a number of enzymes and transporters that are characteristic of such enterocytes: peptidases, esterases, P-glycoprotein, uptake transporters for amino acids, bile acids, carboxylic acids, etc.

How long does it take for Caco-2 cells to adhere?

Although short time protocols for obtaining a polarized Caco-2 cell monolayer has been published, the standard and most reliable procedure requires 21 days of culture to obtain a polarized monolayer with full expression of tight junctions and other intercellular contacts.

What is the Caco?

A Casualty Assistance Calls Officer (CACO) is the official representative of the Secretary of the Navy who provides information, resources and assistance to the PNOK and SNOK in the event of a casualty.

Are Caco 2 cells adherent?

Early studies revealed that differentiated Caco-2 cells expressed several morphological and functional properties characteristic of small bowel enterocytes….10.2 Features and Mechanisms.